Olympus BX53 Upright Fluorescence Microscope w/ (New Filters) in Walpole, MA, USA

- Includes new fluorescence filters
- Technical sales consultation available
- Continental USA 180 DAYS WARRANTY & 60 DAYS INTERNATIONAL
- Includes remote microscope training session and software overview

Service Highlights

- Optics quality is checked for field distortion and uniformity at the specimen plane
- Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
- Preventive Maintenance (PM) is Completed

More info about our Microscopy Capabilities or view the Microscope Buying Guide
---------------------------------------------------------------

This is what you will receive before customizing this microscope:

Standard Olympus BX53 Upright Fluorescence Microscope Configuration:

Microscopy Techniques:

Brightfield and Fluorescence Microscopy

Microscope Base:

- Olympus BX53F Upright Microscope

Microscope Head:

- Trinocular Head with camera port (please note the adjustable head shown in the photos is optional)
- Two Eyepieces

Fluorescence Light Path:

- Mechanical fluorescence path

Fluorescence Illumination:

- EXFO X-Cite Metal Halide Lamp with collimating adapter and light guide

Fluorescence filters:

- Three filter cubes
- Three Brand New filter sets of either DAPI, FITC, Texas Red or CY3

Brightfield Light Path:

- Base

Brightfield Illumination:

- Halogen Lamp

Objective Turret:

- 6-position mechanical nosepiece

Objectives:

- 4x/0.13 UPlanFL
- 10x/0.30 UPlanFL
- 20x/0.50 UPlanFL

Condenser:

- Mechanical condenser

Stage:

- Mechanical XY with slide holder

Photo/Port Split:

- Eyes: 100%
- Eyes/Camera: 50%/50%
- Camera: 100%

Computer & Software:

- Computer with camera capture software
- ImageJ image analysis software

Accessories:

- Necessary power and communication cables

This configuration includes any 3 of these new filter sets.

- New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
- New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
- New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
- New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
- New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

This microscope's imaging capabilities:

Imaging software highlights:

- Ability to take still images and live videos of your samples
- Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
- Variable linear measurements such as line segment, radius values, and others

We can customize this microscope to fit your requirements:

Add Microscopy Techniques (In addition to installed Brightfield and Fluorescence)

- Phase Contrast, DIC, or Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality:

- Microscope can be configured for magnification between 50x and 100x (with 10x Eyepieces)
- We can add standard higher magnification Olympus microscope objectives
- We can add high-end Olympus Apochromat objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

- Additional filter cubes
- Additional filter sets to match your fluorochromes.
- Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities:

- We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

Add other Motorized/Automation Capabilities:

In addition to installed motorized capabilities, the microscope can be configured

- Motorized Stage, Motorized high-speed shutter, Motorized filter cube turret

We can configure it to work with the sample medium you need:

- Slide, Petri dish, microplate, etc.
- It may also be set up with a motorized XY stage for multi-slide or full microplate scans.

Commonly Requested Customer Upgrades

- High-End CCD, sCMOS Cameras
- LED or Metal Halide Fluorescence Light Sources
- Phase Contrast, Polarization, or the DIC configurations
- Higher Magnification Objectives
- Higher performance Fluor or APO objectives
- Motorized XY Stage (stage, controller, joystick)

We are excited to help build and configure the right microscope for you!

Olympus BX53 Upright Fluorescence Microscope Overview

(Final microscope capabilities will depend on the modifications you request)

- The Olympus BX53 is a modular upright microscope designed for both brightfield and fluorescence microscopy applications.
- It features a U-TTR-2 trinocular head and an 8-position BX3-RFAS mechanical fluorescence light path.
- Fluorescence illumination is delivered by an EXFO X-Cite metal halide lamp with a collimating adapter and liquid light guide.
- The system includes DAPI, FITC, Texas Red, or CY3 filter sets and an ND filter slider for fluorescence control.
- Objectives include 4x, 10x, and 20x UPlanFL lenses mounted on a 6-position mechanical nosepiece.
- A mechanical XY stage, U-AC2 condenser, and standard photo port splitting enable versatile imaging and documentation.

Suited for the following applications as configured:

- Fluorescence microscopy: Ideal for fluorescent labeling and imaging of cells and tissues.
- Material science research: Suitable for examining material properties and structures.
- Biological research: Essential for cellular structure, function, and behavior studies.
- Pharmacological studies: Facilitates the investigation of drug effects on cells and tissues.
- Biotechnology applications: Enables genetic engineering and molecular biology research.
- Educational purposes: Serves as a teaching tool in universities and research institutes.

Other potential applications:

- DIC: Enhances visibility of transparent specimens without staining.
- High-resolution imaging: Provides clear, detailed images for in-depth analysis of samples.
- Phase contrast: Enhances visibility of transparent specimens without staining.

- Brochure: Olympus BX53 Upright Fluorescence Microscope brochure
- Manual: Olympus BX53 Upright Fluorescence Microscope manual

Check out our microscopy capabilities:

https://www.bostonind.com/our-microscopy-capabilities

REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US

- They want to ensure the microscope is customized to fit their application
- They want to ensure that internal optics are properly cleaned
- They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
- They want to ensure that image quality is verified at the sample point for field distortion and uniformity
- They want to ensure that the fluorescence intensity is verified at the sample point
- They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
- They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
- They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
- They want training to learn how to operate the microscope
- They want a reliable warranty
- They want peace of mind knowing it will be safely and professionally packed for shipping

Our Advantage

- We make sure to match the microscope capabilities to your requirements.
- We have experience servicing and repairing these microscopes.
- This microscope goes through an extensive set of service steps before shipping.
- We offer detailed photos and videos of the microscopes you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Many upgrade and repair parts are in stock.
- Remote installation and microscope operation training are available.
- On-site installation may be available in the Boston Area (New England area)
- Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Our Guarantee

- Warranty
- An extended warranty may be available.
- You get after-sales technical support.
- Customer references are available.
- The microscope will be complete and working!
- Smooth process from the initial quote to order fulfillment.

*SM* Small Crate

300#

PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?

A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.

Q: Can you make the necessary modifications to the standard configuration to fit my application?

A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application

Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?

A: Each microscope goes through extensive service to ensure it performs to specification.

Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?

A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?

A: The microscope comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?

A: We offer remote installation and training packages to get you familiar with this microscope

Q: What should I watch out for when buying a used/pre-owned microscope from another lab?

A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives.

Q: Do you have these and other microscopes on hand?

A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.

Q: I am from the University or College, do you accept Purchase Orders?

A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?

A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.

Q: Will my instrument be insured during shipping?

A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: Have you packed and shipped microscopes in the past?

A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.

Q: Do you ship Internationally?

A: So far, we have shipped to almost every continent and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?

A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.